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bcam positive cell isolation  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec bcam positive cell isolation
    Bcam Positive Cell Isolation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcam+positive+cell+isolation/CD239+(BCAM)+Antibody%2C+anti-human%2C+REAfinity/pmc09480518-213-1-14
    Average 93 stars, based on 6 article reviews
    bcam positive cell isolation - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation
    Article Snippet: Cells were washed twice with PBS supplemented with 2% FBS and incubated with 30nM SYTOX Green Dead Cell Stain (Thermo Fisher Scientific) to exclude nonviable cells. .. For BCAM-positive cell isolation, the cells were incubated with 4μg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) and Propidium Iodide Staining Solution (BD Biosciences, San Jose, CA). .. For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5μg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5μg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4μg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.
    Article Snippet: Cells were washed twice with PBS supplemented with 2% FBS and incubated with 30nM SYTOX Green Dead Cell Stain (Thermo Fisher Scientific) to exclude nonviable cells. .. For BCAM-positive cell isolation, the cells were incubated with 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) and Propidium Iodide Staining Solution (BD Biosciences, San Jose, CA). .. For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5mg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5mg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.

    Incubation:

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation
    Article Snippet: Cells were washed twice with PBS supplemented with 2% FBS and incubated with 30nM SYTOX Green Dead Cell Stain (Thermo Fisher Scientific) to exclude nonviable cells. .. For BCAM-positive cell isolation, the cells were incubated with 4μg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) and Propidium Iodide Staining Solution (BD Biosciences, San Jose, CA). .. For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5μg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5μg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4μg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.
    Article Snippet: Cells were washed twice with PBS supplemented with 2% FBS and incubated with 30nM SYTOX Green Dead Cell Stain (Thermo Fisher Scientific) to exclude nonviable cells. .. For BCAM-positive cell isolation, the cells were incubated with 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) and Propidium Iodide Staining Solution (BD Biosciences, San Jose, CA). .. For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5mg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5mg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.

    Staining:

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation
    Article Snippet: Cells were washed twice with PBS supplemented with 2% FBS and incubated with 30nM SYTOX Green Dead Cell Stain (Thermo Fisher Scientific) to exclude nonviable cells. .. For BCAM-positive cell isolation, the cells were incubated with 4μg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) and Propidium Iodide Staining Solution (BD Biosciences, San Jose, CA). .. For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5μg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5μg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4μg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.
    Article Snippet: Cells were washed twice with PBS supplemented with 2% FBS and incubated with 30nM SYTOX Green Dead Cell Stain (Thermo Fisher Scientific) to exclude nonviable cells. .. For BCAM-positive cell isolation, the cells were incubated with 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec, Bergisch Gladbach, Germany) and Propidium Iodide Staining Solution (BD Biosciences, San Jose, CA). .. For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5mg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5mg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.

    other:

    Article Title: Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.
    Article Snippet: For co-labeling of ABCB5 and BCAM in freshly isolated limbal epithelial cells, the cells were incubated with 2.5mg/ml mouse anti-ABCB5 monoclonal antibody (mAb) (clone 3C2-1D12) conjugated with Alexa Fluor 647, 0.5mg/ml Brilliant Violet 421-conjugated CD45 mAb (clone 2D1, BioLegend), 4mg/ml VioBright FITC-conjugated anti-BCAM mAb (Miltenyi Biotec) and Propidium Iodide Staining Solution.



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    Miltenyi Biotec bcam positive cell isolation
    Bcam Positive Cell Isolation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcam+positive+cell+isolation/CD239+(BCAM)+Antibody%2C+anti-human%2C+REAfinity/pmc09480518-213-1-14
    Average 93 stars, based on 1 article reviews
    bcam positive cell isolation - by Bioz Stars, 2026-09
    93/100 stars
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